How to Choose Buffer for Gel Electrophoresis

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12 Mar 2023

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Choosing the optimal running buffer for electrophoresis Should you use TAE or TBE running buffer? TAE - Longer fragments are better resolved when TAE buffer is used (usually for fragments over 1 kb) Compatible with enzymatic reactions Recommended for preparative gel electrophoresis TBE - Commonly used for better separation of small DNA fragments #NikolaysGeneticsLessons #gelElectrophoresis #gelElectrophoresisExplained #gelElectrophoresisOfDna #gelElectrophoresisProcedure #gelElectrophoresisLab #gelElectrophoresisAnalysis #agaroseGelElectrophoresis #agaroseGelElectrophoresisProtocol #agaroseGelElectrophoresisOfDna #agaroseGelElectrophoresisExplained #dnaGelElectrophoresis #dnaElectrophoresis #electrophoresisOfDna #sdsPageGelElectrophoresis #polyacrylamideGelElectrophoresis #glycerol #TBE

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