How to calculate concentration of TBE or Tris/Borate/EDTA buffer

TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA. In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. EDTA is a chelator of divalent cations, particularly of magnesium (Mg2 ). As these ions are necessary co-factors for many enzymes, including contaminant nucleases, the role of the EDTA is to protect the nucleic acids against enzymatic degradation. But since Mg2 is also a co-factor for many useful DNA-modifying enzymes such as restriction enzymes and DNA polymerases, its concentration in TBE or TAE buffers is generally kept low (typically at around 1 mM). More recently discovered substitutes for TBE and TAE buffers for electrophoresis are available. Problem: You have a 10x TBE buffer. To run a gel, you need 500 ml of a 2x solution of TBE. How do you make a 500 ml solution of 2x TBE buffer from the 10x buffer? #TBE #Tris #Borate #TAE #gelElectrophoresis #laboratoryCalculations #dilution #Solution #buffer #chemistry #Genetics #geneticsClass12 #GeneticsLecture #geneticsBiology #geneticsCrashCourse #geneticsAndHeredity