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This single-celled protozoan was imaged using a Zeiss Universal microscope fitted with a Nikon D4 camera set to 30 fps @ 1080p resolution. The cell membrane of the organism ruptures at about 0:40 but then appears to repair the damaged membrane and continue on, unaffected. The objective was a 40x Zeiss Ph3 .8-1.0/na oil immersion planapo with iris. The photo-eyepiece was a Zeiss wide field. The Universal’s Optovar magnification changer was set to (40 x 12.5 x = 625x) The iris was stopped down to .8/na and the scope was setup for phase contrast viewing. I’ve had many requests to explain what I mean when I refer to “darkfield-phase contrast lighting“. With the objective iris of the 40x Zeiss Ph3 .8-1.0/na oel planapo with iris stopped down to .8/na and the scope aligned for phase contrast lighting the central dark area of the phase annuli in the condenser effectively functions as a darkfield stop and results in the black background while the alignment of the phase rings in the objective and
