This is a tool-assisted speedrun. For more information, see TAS originally published on 2005-10-06. Here you can see a revision of this game by nitsuja (), 4: (16,519 Frames @60 FPS) minutes faster than the previous run by mazzeneko. The strategy used was changed radically, using Raphael instead of Donatello and using dash moves more often. The damage for every move was mapped out carefully by monitoring the internal values of the RAM, to be able to choose the best attack for every situation. Luck was manipulated to make some enemies appear in more convenient positions.
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