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The Western blot protocol provides scientists with a means of comparing relative protein levels in different samples. Proteins are differentiated by size on an acrylamide gel and visualized on a membrane with antibodies. Learn more about how this protocol allows us to determine what proteins are present in a cell and under what conditions. This video is part of the Research Integrated Science Education (RISE) Program that is funded by SENS Research Foundation and Dalio Philanthropies. The program is designed to introduce high school students to aging research and laboratory concepts, such as experimental design and data interpretation, within core curriculum standards. To learn more about RISE and to access additional functionalities such as the transcript glossary function, visit
