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DNA agarose gel electrophoresis is an analytical method used to separate DNA fragments by length. It is based on the different speed of movement of fragments of different lengths when moving in the gel under the action of an external electric field. One of the applications of the method is the study of plasmid DNA. It is usually annular and forms secondary structures, such as coiling into a supercoil. Thus, to determine the size of the plasmid, it is necessary to destroy the elements of the secondary structure. To do this, before applying to the gel, the plasmid is “linearized” (make it linear), that is, it is treated with one of the restriction enzymes (endonucleases). The restriction enzyme is chosen so that it cuts the plasmid in only one place. To separate DNA fragments of different lengths, a gel with different concentrations of agarose is used. The shorter the length of the DNA fragments to be separated, the greater the concentration of agarose should be. Problem: You want to make a 0.5% agarose gel. How much agarose (in grams) do you need to make up a 50 ml gel solution? #gelElectrophoresis #laboratoryCalculations #ratio #proportion #Dna #biology #gelElectrophoresisExplained #gelElectrophoresisOfDna #gelElectrophoresisProcedure #gelElectrophoresisLab #gelElectrophoresisAnalysis #agaroseGelElectrophoresis #agaroseGelElectrophoresisProtocol #agaroseGelElectrophoresisOfDna #agaroseGelElectrophoresisExplained #dnaGelElctrophoresis #dnaElectrophoresis #electrophoresisOfDna
