Confrence - E. CHARPENTIER - Le CRISPR Cas9 - La rvolution de l'ingnierie gnomique

Conférence de Emmanuelle CHARPENTIER intitulée The CRISPR-Cas9 revolution in genome engineering: lessons learned from bacteria 00:00:00 Introduction 00:01:10 Understand diseases at the fundamental level 00:04:16 Jacques Monod (1910-1976) 00:05:30 Important milestones towards gene therapy 00:10:09 The CRISPR craze 00:10:56 Bacterial pathogens 00:11:26 Understand bacteria/host interactions 00:11:52 Infection of bacteria by invading genomes 00:14:14 The CRISPR-Cas adaptive immune system 00:17:37 - Spacer acquisition 00:19:04 - crRNA expression and maturation 00:20:06 - interference 00:23:01 - mecanisms 00:23:06 CRISPR-Cas has evolved into five major types 00:24:02 Sans titre 00:26:19 CRISPR-Cas9 technology: a two-component system 00:29:04 CRISPR-Cas9 pathway in bacteria 00:29:09 Type II CRISPR-Cas locus 00:29:30 Type II CRISPR-Cas: model of crRNA maturation 00:30:22 Type II CRISPR-Cas: model of DNA targeting 00:30:38 Type II CRISPR-Cas