The role of Glycerol in Gel Electrophoresis

The role of Glycerol: You might be thinking that I had not mentioned glycerol and we are not using it in electrophoresis, right! The role of glycerol is very very important to run a DNA gel. DNA can diffuse in a running buffer and comes out from wells. We need to sink it on the bottom of the gel so that it can pass through gel pores. Glycerol gives density to DNA and makes it heavier than the buffer. By using glycerol, DNA remains at the bottom of the gel well. But you don’t need to add it separately. It is already present in the loading dye. With BPB or orange G, the glycerol is added, already. Usually, 2.5 to 3% glycerol is added. #NikolaysGeneticsLessons #gelElectrophoresis #gelElectrophoresisExplained #gelElectrophoresisOfDna #gelElectrophoresisProcedure #gelElectrophoresisLab #gelElectrophoresisAnalysis #agaroseGelElectrophoresis #agaroseGelElectrophoresisProtocol #agaroseGelElectrophoresisOfDna #agaroseGelElectrophoresisExplained #dnaGelElectrophoresis #dnaElectrophoresis #electrophoresisOfDna #sdsPageGelElectrophoresis #polyacrylamideGelElectrophoresis #glycerol