I sacrificed a finger to bring you this video. please subscribe Join the Plants in Jars discord server! Where I buy tissue culture supplies: (use code PLANTSINJARS for 10% off) Find me on Instagram: Supplies List Below! • Scale ( accuracy): • pH Meter ( pH accuracy): • pH Up & Down: • Spray Bottle: • Plastic Gloves: • Forceps: • Scissors: • Sterile Petri Dishes: • mL Droppers: • Agar: • Murashige & Skoog: • Kinetin: • Polypropylene Containers: • Light Meter: Protocol for video: (I am aware that Philodendron Mamei is not technically a “self heading philodendron“, but this protocol has worked well for me before for other similar philos!) Timestamps: • 1:31 Building a still air box • 3:18 Tissue culture supplies • 4:45 What is a tissue culture protocol? • 5:03 Where to find tissue culture protocols • 5:44 What is tissue culture media? • 8:55 How to read a tissue culture protocol • 11:00 Making tissue culture media • 13:52 Sterilizing the media • 17:19 Prepping the still air box • 18:30 Preparing the explants (tissue samples) • 19:39 Putting the explant into tissue culture • 25:30 Conditions for growth • 26:19 When to subculture Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.
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