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Proteomics involve the large-scale study of proteins, their structure and physiological role or functions. Proteins are quintessential cellular components or biomolecules in any living organism. The term proteomics first appeared in 1997. It was very similar to the word genome. The word proteome is actually a combination of protein and genome and was coined by Mark Wilkins in 1994. To be precise and specific, proteome is the entire complement or database or set of proteins produced by a living organism. The proteome is a broad term that also encompasses the alterations or modifications produced in native protein when organisms are subjected to a plethora of changes. Genome walking - is a method for determining the DNA sequence of unknown genomic regions flanking a region of known DNA sequence. The Genome walking has the potential to capture 6-7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region. Chromosome walking is a method of positional cloning used to find, isolate, and clone a particular allele in a gene library. • Chromosome Walking was developed by Welcome Bender, Pierre Spierer, and David S. Hogness in the Early 1980's. Gene mapping - describes the methods used to identify the locus of a gene and the distances between genes. The essence of all genome mapping is to place a collection of molecular markers onto their respective positions on the genome. Molecular markers come in all forms. #Geneics #Proteomics #DNA #proteins #NikolaysGeneticsLessons #protein #polypeptide #aminoAcid #peptide #disulfideBridges #covalentBounding #ionicBounding #hydrophobicInteraction #proteinStructure #proteinSequence #proteinFolding #aminoAcids #Polypeptides #ImidazolRing #Histidine #polypeptideChain #oligopeptide #hydrolysesSynthesis #condensationReaction #condensationSynthesis #fibrousProteins #genetics
